• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy
【2h】

Anabolism versus catabolism of [5-3H]uridine and its relationship to ribonucleic acid labelling in mouse liver after partial hepatectomy

机译：肝部分切除术后[5-3H]尿苷的合成代谢与分解代谢及其与核糖核酸标记的关系

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The balance between anabolism and catabolism of [5-3H]uridine was studied in the mouse after partial hepatectomy. Labelling of RNA and UDP-glucose was determined and evaluated in relation to changes in the specific radioactivity of UTP. The amounts of labelled catabolic products of uridine were increased several-fold in liver and blood after partial hepatectomy. The specific radioactivity of RNA decreased to about 60% of the control value at 6h and was in the same range as that of control liver at 24h after operation. Decreased labelling of RNA and UDP-glucose was attributable to decreased specific radioactivity of UTP. No changes in the size of the UTP pool or in the balance between uridine anabolism and catabolism were found that could explain the decreased specific radioactivity of UTP. Rather, the alterations in the labelling of this metabolite induced by the partial hepatectomy may be related to decreased phosphorylating capacity in the liver cells and/or dilution of the labelled precursor in an expanded uridine pool. The enhanced amounts of uridine catabolic products in liver and blood were probably a consequence of accumulation and altered incorporation of the metabolites from the blood into the liver cells. Despite the increased amounts of labelled catabolic products and the decreased labelling of RNA, the results reported here actually suggest decreased uridine catabolism and slightly increased RNA synthesis in mouse liver after partial hepatectomy. The results stress the importance of proper controls in determination of nucleic acid synthesis and in metabolic studies by use of labelled precursors.
机译：在部分肝切除术后的小鼠中研究了[5-3H]尿苷的合成代谢与分解代谢之间的平衡。确定了RNA和UDP-葡萄糖的标记，并根据UTP特定放射性的变化进行了评估。部分肝切除后，肝脏和血液中尿苷标记的分解代谢产物的含量增加了几倍。术后6h RNA的比放射性降至对照值的60％左右，与术后24h的对照肝处于相同范围。 RNA和UDP-葡萄糖的标记减少归因于UTP的比放射性降低。没有发现UTP池的大小变化或尿苷合成代谢和分解代谢之间的平衡变化，可以解释UTP比放射性降低。而是，由部分肝切除术引起的该代谢产物的标记的改变可能与肝细胞中磷酸化能力的降低和/或在扩大的尿苷池中标记的前体的稀释有关。肝脏和血液中尿苷分解代谢产物量的增加可能是血液中代谢物积累和改变并入肝细胞的结果。尽管标记的分解代谢产物的量增加且RNA的标记减少，但此处报道的结果实际上表明，部分肝切除术后小鼠肝脏的尿苷分解代谢降低，RNA合成略有增加。结果强调了通过使用标记的前体在核酸合成测定和代谢研究中进行适当控制的重要性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号